Raman spectroscopy can provide easy and quick information of sample.It is useful to determine the vibrational energy of sample molecule as well as rotational and other low energy molecules.In this technique laser light is used for scanning of the sample. Raman spectroscopy is very useful and qualitative technique. When the laser light is interact with the molecular vibration, photons and other excitation, resulting in the energy of the laser photons is shifted to up or down. Raman spectroscopy is depends on the inelastic scattering of photons, this effect is known as Raman scattering. A proper detector also very important factor. Spectrometer is an important performance factors are small form factor, low power consumption, high resolution, less time and low noise. The fiber optic probe is easily change the microscopes, gas flow cells, liquid flow cells and remaining sampling parts. In spectrometer Fiber optic probe is typically used that provide a maximum sampling interface. Raman spectroscopy instrumentationīasically Raman Spectroscopy includes three main components first is Laser, sampling interface and spectrometer. Stoke scattering: When shift at wavelength is higher than that of the incident light is called as Stoke scattering.Īntistoke Scattering: When shift at wavelength is lower than that of the incident light is called as antistoke scattering. It includes Stoke scattering and anti stoke scattering. The Raman shift is having two different energy bands. Raman shift is a difference in energy between incident light and scattered light. “Change in the wavelength of light that occurs when a light beam is deflected by sample molecule.” Raman effect includes elastic scattering at the same wavelength as the incident radiation and inelastic scattering at the different wavelength due to molecular vibrations. When light transferred from the sample, the lights are scattered in multiple directions. ” Frequency of small fraction of scattered light is different from frequency of monochromatic incident light.” It is qualitative technique to determine functional group of the drug. Raman spectroscopy is a analytical technique to determine the vibrational energy modes of sample with the help of scattered light. Raman spectroscopy is based on light scattering technique. Difference between Raman Spectroscopy and IR Spectroscopy.Here we will see, Raman spectroscopy uses advantages and disadvantages in pharmaĪpart from that remaining points also will cover such as, Sir C.V Raman is basically Tiruchirapalli belong to Tamilnadu state of India. Raman) was born on 7 November 1988 and died on 21 November 1970. Raman also awarded by Indian government by the Bharat Ratna award in 1954. Raman is the first asian person who awarded by Nobel prize. Raman awarded with Nobel prize in physics in 1930 for his invention. The name Raman spectroscopy is given on the honour of Sir C.V. Raman Spectroscopy is invented by the Indian physicist Sir C.V. Difference between Raman spectroscopy and IR Spectroscopy Raman spectroscopy uses advantages and disadvantages in pharma.
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